Hi overmyhead,
I understand that you are trying to write to only certain lines in your port, using a line mask. To do this, you will simply want to specify a value for the "line mask" input of the DIO Port Write VI. This input is an I32 value; thus you can simply input a value of "224" which would represent the value "11100000" in binary (bits 5, 6, and 7 are set to '1' to indicate that these lines will be written to). I am not sure what cluster you are referring to. Many times, a cluster or array of boolean values is used to represent bits, and this cluster or array is then converted to an integer value.
For an example of writing values to a single line within a port, please refer to the LabVIEW Example Finder (under Help » Find Examples). Within the Example Finder, if you choose to browse according to directory structure, you can find this example under "daq » digital » E-series.llb » Write 1 Pt to Dig Line (E).vi". While this example may not be exactly the same as what you are trying to do, it gives a good idea of how the DIO Port functions are used. I hope this helps,
Daniel S.
National Instruments