Hi Mama,
The calibration interval is based on time between hardware calibrations, so if you require the 4060 to be guaranteed within the accuracy specifications defined here:
you will need to apply the hardware configuration, even if the card hasn't been in use. As you will see, the specifications give figures for absolute accuracy at different time intervals after calibration, regardless of whether the device has been used or not.
I hope this helps answer your question.
Best wishes,
Mark
Applications Engineer
National Instruments