I'm trying to make serial communication between a computer and RTE 140 Bath/Circulator (from NESLAB). When I use "serial communication.vi", it works well. I can write to and read from the instrument. Since I want to write from a file, I replaced sub vi, "VISA Write.vi" in "Serial communication.vi", with sub vi "VISA Write from File.vi". Then, I run this new vi and sent two commands to the instrument (one is set setpoint and another one is read setpoint). It works for "set setpoint"(I got "!"), but I got ? on "read setpoint". I appreciate if anyone can help.