The dt input on the Timed Loop should be equal to the number of samples you are reading during each iteration. For example, if you were sampling at 1000 Hz and reading 200 samples during each iteration of the loop, dt should be set to 200. Notice that because you've wired the external timing source to the Timed Loop, the Timed Loop is looking for dt in terms of ticks of the sample clock, in this case.
According to your task, you are reading 250k samples and sampling at 250kHz. So, you would probably try a dt of 250000 ticks. However, you'll probably get error -200279 from DAQmx, which means you need to read the data more frequently.
If you have more questions, please post a reply.
Regards,
Kristi H
National Instruments