Vision Assistant Loses Calibration After "Subtract" Step

[visionmotion]

I have a Vision Assistant script that calibrated the captured image to real world units (mm).  This worked great in outputing, for example, the real-world coordinates of circles inside the given image.  However, once I perform a "subtract" operation, subsequent circle matches are void of calibrated data, showing only the coordinates in pixels.

Any rhyme or reason for this?  Looking for a solution to provide calibrated output data.

[visionmotion]

Could it be related to this? http://digital.ni.com/public.nsf/websearch/5FC615E2B4CFE8FA86256AC4007C9A42?OpenDocument I see no options regarding "Extracted Mask Region"

[BruceAmmons]

I am going to guess you are using your calibration image as one of the images in your subtraction step.  Another possibility is that one of the images you are using in your subtraction step is not calibrated.  Either of these might make the image lose its calibration.  The second is more likely, I would think.

Try reapplying the calibration after the subtraction step.

Bruce