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Can I Do Video Analysis With the VISION BUILDER AI 3.5 Or LabVIEW 2009 ?With machine vision

When water molecules freezing , its characteristic value (such as its shape or color) will be changed.Now I want to detect and record these characteristic values.Can I use VISION BUILDER the AI ​​3.5 to fininsh it (Then I want to translate it into LabVIEW)?During water molecules  freezing,each molecule will be changed,so how to set ROI, or use the  template matching? In addition to the gray scale, which characteristic values ​​can be detect?  Thank you.

The video file is very large so in the Attachment,  the duration is very short (only four frame).

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